is sometimes not the most ideal situation: a microscope camera with low resolution, large botanical specimens and a small lens with a 4X magnification, with which it is not possible for a whole product to survey.
addition frustrated because the microscope can not make using the 4x objective, the field of view evenly lit off.
The experiment from several individual images that overlap could lead to create an overall picture of the solution.
landscape photographer beat has long been around with this problem if it is to create panoramic photos, and so there are a lot of software that helps to create panoramic photos.
After some experimentation, I finally stopped at Autostitch hang . Actually, I was skeptical about the final results, as individual images for panorama almost always from a fixed position made be made to rotate the camera while shooting, while the other hand, the lens of the microscope on the specimen and moves for each photo takes a different point of observation (so-called multi-view images).
But too little biased by the whole theory for panoramic landscapes and the projection of possibilities and problems I rolled up his sleeves and began my blue-eyed experimentation. showed
Autostitch is a program for the Window operating system, but initial tests soon: It works fine on wine on Linux.
The first hurdle would have done it.
are now the single shots produce - but some basic requirements should be given for it: A really smooth running XY stage, a constant source of bright light and a static product - from a fidgety ant it will be hard to get matching frames that will later fit together.
In addition, it should be possible with the recording software of the camera to automatically periodically to take pictures - this makes the work immensely, when just more focus must be on the next shot and not have to press the shutter button.
is first recorded the first series, the individual images have to overlap so that composed Autostitch the images later can automatically - the overlapping area between two adjacent images should be at least 30-40%:
and now the second row - this of course also up from the first set overlap:
and now the third row, which has up again with the second row overlap:
and so on and so on ...
now be given the post - some photos are double or blurred.
Double and blurred photos must be deleted or should they move, at least in a different directory. However, what
Note: To obtain a good overall picture, it must! a complete series of still images available, each of which overlap with their neighbors!
After finishing you can now start the program Autostitch.
are first change to OPTIONS some settings, since we've optimized Autostitch for panoramic pictures and not for "Multi View" series so some adjustment is likely:
Highlights:
Output Size : Here we define the size of the final image.
Note: An image can get fast times 50mpx and consume correspondingly large amount of RAM on your computer. In the worst case, the program will fail Autostitch leaks memory.
Rendering Options : Autostitch glue the frames together, but rather generated from the data of the individual images, a new picture.
What method is used to set you can here - MultiBanc yields the best results, this method is very memory hungry and if you have too many individual images (in my 3Mpx recordings here, the limit is at 50-60 images), then You have to resort to linear , with this setting it is I already managed to hang together 260Einzelbilder.
GainCompensatior : The frames are different will be slightly in brightness - Autostitch to correct the differences in brightness, then you should select this option, which never really hurt.
CropSettings : AutoCrop here is actually more useful.
matching options : Autostitch uses complex algorithms to determine which are the overlapping images together. Here you can engage in the operation of the algorithm. The above settings applied after a few tries but the solution to an overall picture of 260Einzelbildern create.
OrientationSettings : can disable auto straighten - the microscope photographs are usually recorded under better conditions than panoramic photos at the top free hand in high winds and are therefore virtually all strictly parallel to each other, this correction should not really be necessary.
other options: Here you define how much RAM can use Autostitch - many still images can Autostitch ever use the 2 GByte RAM.
addition we define here the quality of the final image - you choose 100%.
In FILE and OPEN to Choose the frames from now - can Autostitch need here on a 2GHz machine with 2 GByte of RAM at 260 frames for each 3Mpx ever in the two hours.
Autostitch is successful, the final result is obtained under the name stored pano.jpg.
Autostitch is used in Linux is obtained on the command line of the message "Access Denied " that Linux is also trying to launch an image viewer like in Windows in order to present the final result.
This launch attempt fails, however, Linux and leads to this error message.
The end result should, however, just before pano.jpg as in Windows, located in the file.
If one has a finished picture there be a need to deliver it in an image editing program in form since the end result is still surrounded by a black border and The white balance was perhaps not optimally adjusted to the camera.
might want to shrink the bottom line yet, because images can be present with 80mpx.
Here is a root cross-section of Allium ursinum - released final results from 56 individual images:
You can see very well the limits of the individual images. To fill the black area, is of course very good image editing program gimp.
gimp can also download for Windows, but feels really good only on Linux.
In gimp there is the tool wand "- meaning a similar Select an area with the same color.
So fill " wand " and then click in the black area, then the selected area with " fill " with white paint:
Now it is obvious - the white balance on the camera was not optimal - ie gimp must help again: In
gimp to " colors", click on " values" - opens> a new window, among other things in it are three buttons to see an eyedropper icon in it. In one of these buttons is
it " White Point Select ", click on it, the cursor in the gimp is a small pipette, so you click in the gray area and get:
Note: When you click the eyedropper and it does nothing in the picture , then check whether there are a range is selected. In this case, " selection" and then "Select None .
The remaining shadow you can then use the magic wand , eraser, etc. in ! remove gimp
Granted some work - but the end result is worth many times the efforts
Other examples:
See! my collection of pictures (all photos are there under the Creative Common License - released Attribution ShareAlike ):
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| photomicrographs Botany |
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